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Journal article

Noncatalytic ions direct the RNA-dependent RNA polymerase of bacterial double-stranded RNA virus ϕ6 from de novo initiation to elongation.

Abstract:

RNA-dependent RNA polymerases (RdRps) are key to the replication of RNA viruses. A common divalent cation binding site, distinct from the positions of catalytic ions, has been identified in many viral RdRps. We have applied biochemical, biophysical, and structural approaches to show how the RdRp from bacteriophage ϕ6 uses the bound noncatalytic Mn(2+) to facilitate the displacement of the C-terminal domain during the transition from initiation to elongation. We find that this displacement rel...

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Publication status:
Published

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Publisher copy:
10.1128/jvi.05168-11

Authors


More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
Structural Biology
Role:
Author
Journal:
Journal of virology
Volume:
86
Issue:
5
Pages:
2837-2849
Publication date:
2012-03-01
DOI:
EISSN:
1098-5514
ISSN:
0022-538X
Source identifiers:
222350
Language:
English
Keywords:
Pubs id:
pubs:222350
UUID:
uuid:9f06cc6b-d52b-4a93-93c1-370b3cb0daf1
Local pid:
pubs:222350
Deposit date:
2012-12-19

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