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Noncatalytic ions direct the RNA-dependent RNA polymerase of bacterial double-stranded RNA virus ϕ6 from de novo initiation to elongation.

Abstract:

RNA-dependent RNA polymerases (RdRps) are key to the replication of RNA viruses. A common divalent cation binding site, distinct from the positions of catalytic ions, has been identified in many viral RdRps. We have applied biochemical, biophysical, and structural approaches to show how the RdRp from bacteriophage ϕ6 uses the bound noncatalytic Mn(2+) to facilitate the displacement of the C-terminal domain during the transition from initiation to elongation. We find that this displacement rel...

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Publication status:
Published

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Publisher copy:
10.1128/jvi.05168-11

Authors


Poranen, MM More by this author
Bamford, DH More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Structural Biology
Journal:
Journal of virology
Volume:
86
Issue:
5
Pages:
2837-2849
Publication date:
2012-03-05
DOI:
EISSN:
1098-5514
ISSN:
0022-538X
URN:
uuid:9f06cc6b-d52b-4a93-93c1-370b3cb0daf1
Source identifiers:
222350
Local pid:
pubs:222350

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