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Regulatable vectors for environmental gene expression in Alphaproteobacteria.

Abstract:
Two expression vectors utilizing the inducible taurine promoter (tauAp) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta. The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.

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Publisher copy:
10.1128/aem.01188-12

Authors


Rudder, SJ More by this author
Bourdès, A More by this author
Karunakaran, R More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Plant Sciences
Journal:
Applied and environmental microbiology
Volume:
78
Issue:
19
Pages:
7137-7140
Publication date:
2012-10-05
DOI:
EISSN:
1098-5336
ISSN:
0099-2240
URN:
uuid:9e4c19af-9864-45ec-9a21-180c318a60aa
Source identifiers:
411923
Local pid:
pubs:411923

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