Functional redundancy of promoter elements ensures efficient transcription of the human 7SK gene in vivo.

Journal article

Deletion and mutation studies of the human 7SK gene transfected into HeLa cells have identified three functional regions of the promoter corresponding to the TATA box at -25, the proximal sequence element (PSE) between -49 and -65 and the distal sequence element (DSE) between -243 and -210. These elements show sequence homology to equivalent regions in other snRNA genes and are functionally analogous. Unlike the DSEs of many snRNA genes however, the 7SK DSE does not contain a consensus bindin...

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Authors: Boyd, D; Turner, P; Watkins, N; Gerster, T; Murphy, S

• Publication status: Published
• Journal: Journal of molecular biology
• Volume: 253
• Issue: 5
• Pages: 677-690
• Publication date: 1995-11-01
• DOI: 10.1006/jmbi.1995.0582
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: Humans; Animals; Host Cell Factor C1; Transcription Factor TFIID; DNA-Binding Proteins; Macromolecular Substances; Sequence Deletion; Xenopus Proteins; Base Sequence; Oocytes; Amino Acid Sequence; Molecular Sequence Data; Hela Cells; RNA Polymerase II; TATA Box; Promoter Regions, Genetic; Xenopus laevis; RNA, Small Nuclear; RNA Polymerase III; Transcription, Genetic; Transfection; Octamer Transcription Factor-1; Mutagenesis; Recombinant Fusion Proteins; Transcription Factors; Enhancer Elements, Genetic