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Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads

Abstract:

Replication of eukaryotic genomes is highly stochastic, making it difficult to determine the replication dynamics of individual molecules with existing methods. We report a sequencing method for the measurement of replication fork movement on single molecules by detecting nucleotide analog signal currents on extremely long nanopore traces (D-NAscent). Using this method, we detect 5-bromodeoxyuridine (BrdU) incorporated by Saccharomyces cerevisiae to reveal, at a genomic scale and on single mo...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted Manuscript

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Publisher copy:
10.1038/s41592-019-0394-y

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ORCID:
0000-0002-7422-5582
Spingardi, P More by this author
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Institution:
University of Oxford
Division:
Medical Sciences Division
Department:
NDM
Subgroup:
Target Discovery Institute
ORCID:
0000-0002-8160-2446
Kriaucionis, S More by this author
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Publisher:
Springer Nature Publisher's website
Journal:
Nature Methods Journal website
Volume:
16
Pages:
429-436
Publication date:
2019-04-22
Acceptance date:
2019-03-18
DOI:
EISSN:
1548-7105
ISSN:
1548-7091
Pubs id:
pubs:993651
URN:
uri:9daa2749-230d-4c65-ad5c-4e45d287fd14
UUID:
uuid:9daa2749-230d-4c65-ad5c-4e45d287fd14
Local pid:
pubs:993651
Language:
English
Keywords:

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