- Abstract:
-
Replication of eukaryotic genomes is highly stochastic, making it difficult to determine the replication dynamics of individual molecules with existing methods. We report a sequencing method for the measurement of replication fork movement on single molecules by detecting nucleotide analog signal currents on extremely long nanopore traces (D-NAscent). Using this method, we detect 5-bromodeoxyuridine (BrdU) incorporated by Saccharomyces cerevisiae to reveal, at a genomic scale and on single mo...
Expand abstract - Publication status:
- Published
- Peer review status:
- Peer reviewed
- Version:
- Accepted Manuscript
- Files:
-
-
(pdf, 4.8mb)
-
- Publisher copy:
- 10.1038/s41592-019-0394-y
-
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- Publisher:
- Springer Nature Publisher's website
- Journal:
- Nature Methods Journal website
- Volume:
- 16
- Pages:
- 429-436
- Publication date:
- 2019-04-22
- Acceptance date:
- 2019-03-18
- DOI:
- EISSN:
-
1548-7105
- ISSN:
-
1548-7091
- Pubs id:
-
pubs:993651
- URN:
-
uri:9daa2749-230d-4c65-ad5c-4e45d287fd14
- UUID:
-
uuid:9daa2749-230d-4c65-ad5c-4e45d287fd14
- Local pid:
- pubs:993651
- Language:
- English
- Keywords:
- Copyright holder:
- Müller et al.
- Copyright date:
- 2019
- Notes:
-
© The Author(s), under exclusive licence to Springer Nature America, Inc. 2019
This is the accepted manuscript version of the article. The final version is available online from Springer Nature at: https://doi.org/10.1038/s41592-019-0394-y
Journal article
Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads
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