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Ultra-high resolution 3D imaging of whole cells.

Abstract:

Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50-80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of Whole-cell 4Pi Single-Molecule Switching Nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging...

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Publication status:
Published
Peer review status:
Peer reviewed
Version:
Accepted manuscript

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Publisher copy:
10.1016/j.cell.2016.06.016

Authors


Sirinakis, G More by this author
Allgeyer, ES More by this author
Schroeder, LK More by this author
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James Hudson Brown—Alexander Brown Coxe More from this funder
The Denmark-America Foundation More from this funder
Civilingeniør Frants Allings Legat More from this funder
Knud Højgaards Fond More from this funder
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Publisher:
Elsevier Publisher's website
Journal:
Cell Journal website
Publication date:
2016-07-05
DOI:
EISSN:
1097-4172
ISSN:
0092-8674
URN:
uuid:9d28bf4b-cabb-4f25-94f4-35c709b145aa
Source identifiers:
633521
Local pid:
pubs:633521
Language:
English

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