Journal article
Ultra-high resolution 3D imaging of whole cells
- Abstract:
- Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50–80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 6.2MB, Terms of use)
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(Supplementary materials, zip, 293.0MB, Terms of use)
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- Publisher copy:
- 10.1016/j.cell.2016.06.016
Authors
+ Cancer Research UK
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- Funder identifier:
- https://ror.org/054225q67
- Grant:
- C6946/A14492
+ National Institutes of Health
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- Funder identifier:
- https://ror.org/01cwqze88
- Grant:
- R01 HL124402
+ Howard Hughes Medical Institute
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- Funder identifier:
- https://ror.org/006w34k90
- Publisher:
- Cell Press
- Journal:
- Cell More from this journal
- Volume:
- 166
- Issue:
- 4
- Pages:
- 1028-1040
- Publication date:
- 2016-07-07
- Acceptance date:
- 2016-06-03
- DOI:
- EISSN:
-
1097-4172
- ISSN:
-
0092-8674
- Language:
-
English
- Pubs id:
-
pubs:633521
- UUID:
-
uuid:9d28bf4b-cabb-4f25-94f4-35c709b145aa
- Local pid:
-
pubs:633521
- Source identifiers:
-
633521
- Deposit date:
-
2016-07-14
- ARK identifier:
Terms of use
- Copyright holder:
- Huang et al
- Copyright date:
- 2016
- Rights statement:
- © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
- Licence:
- CC Attribution (CC BY)
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