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Identification of immune correlates of fatal outcomes in critically ill COVID-19 patients

Abstract:
Prior studies have demonstrated that immunologic dysfunction underpins severe illness in COVID-19 patients, but have lacked an in-depth analysis of the immunologic drivers of death in the most critically ill patients. We performed immunophenotyping of viral antigen-specific and unconventional T cell responses, neutralizing antibodies, and serum proteins in critically ill patients with SARS-CoV-2 infection, using influenza infection, SARS-CoV-2-convalescent health care workers, and healthy adults as controls. We identify mucosal-associated invariant T (MAIT) cell activation as an independent and significant predictor of death in COVID-19 (HR = 5.92, 95% CI = 2.49-14.1). MAIT cell activation correlates with several other mortality-associated immunologic measures including broad activation of CD8+ T cells and non-Vδ2 γδT cells, and elevated levels of cytokines and chemokines, including GM-CSF, CXCL10, CCL2, and IL-6. MAIT cell activation is also a predictor of disease severity in influenza (ECMO/death HR = 4.43, 95% CI = 1.08-18.2). Single-cell RNA-sequencing reveals a shift from focused IFNα-driven signals in COVID-19 ICU patients who survive to broad pro-inflammatory responses in fatal COVID-19 -a feature not observed in severe influenza. We conclude that fatal COVID-19 infection is driven by uncoordinated inflammatory responses that drive a hierarchy of T cell activation, elements of which can serve as prognostic indicators and potential targets for immune intervention.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1371/journal.ppat.1009804

Authors


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Role:
Author
ORCID:
0000-0001-8781-2615
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Role:
Author
ORCID:
0000-0002-9694-2216
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Role:
Author
ORCID:
0000-0001-9261-7726
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Role:
Author
ORCID:
0000-0002-1194-2429
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Institution:
University of Oxford
Division:
MSD
Department:
NDM
Sub department:
NDM Experimental Medicine
Role:
Author
ORCID:
0000-0002-6837-8881

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Publisher:
Public Library of Science
Journal:
PLoS Pathogens More from this journal
Volume:
17
Issue:
9
Article number:
e1009804
Place of publication:
United States
Publication date:
2021-09-16
Acceptance date:
2021-07-16
DOI:
EISSN:
1553-7374
ISSN:
1553-7366
Pmid:
34529726


Language:
English
Keywords:
Pubs id:
1195912
Local pid:
pubs:1195912
Deposit date:
2021-11-09

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