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Super-Resolved Traction Force Microscopy (STFM).

Abstract:
Measuring small forces is a major challenge in cell biology. Here we improve the spatial resolution and accuracy of force reconstruction of the well-established technique of traction force microscopy (TFM) using STED microscopy. The increased spatial resolution of STED-TFM (STFM) allows a greater than 5-fold higher sampling of the forces generated by the cell than conventional TFM, accessing the nano instead of the micron scale. This improvement is highlighted by computer simulations and an activating RBL cell model system.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1021/acs.nanolett.6b00273

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
RDM
Sub department:
RDM - Investigative Medicine Division
Role:
Author
Publisher:
American Chemical Society
Journal:
Nano Letters More from this journal
Volume:
16
Issue:
4
Pages:
2633-2638
Publication date:
2016-02-01
Acceptance date:
2016-02-25
DOI:
ISSN:
1530-6992 and 1530-6984
Pmid:
26923775
Language:
English
Keywords:
Pubs id:
pubs:609109
UUID:
uuid:9bcafc29-32da-4fff-b27d-2b05d432d28a
Local pid:
pubs:609109
Source identifiers:
609109
Deposit date:
2017-03-18

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