Journal article

Protocol for separating cancer cell subpopulations by metabolic activity using flow cytometry

Abstract:: Cells, even from the same line, can maintain heterogeneity in metabolic activity. Here, we present a protocol, adapted for fluorescence-activated cell-sorting (FACS), that separates resuspended cells according to their metabolic rate. We describe steps for driving lactate efflux, which produces an alkaline transient proportional to fermentative rate. This pH signature, measured using pH-sensitive dyes, identifies cells with the highest metabolic rate. We then describe a fluorimetric assay of oxygen consumption and acid production to confirm the metabolic contrast between sub-populations.

Publication status:: Published

Peer review status:: Peer reviewed

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APA Style

Blaszczak, W., White, B., & Swietach, P. (2024). Protocol for separating cancer cell subpopulations by metabolic activity using flow cytometry. STAR Protocols, 5(2).

MLA Style

Blaszczak, W., et al. “Protocol for Separating Cancer Cell Subpopulations by Metabolic Activity Using Flow Cytometry.” STAR Protocols, vol. 5, no. 2, Cell Press, 2024.

Chicago Style

Blaszczak, W, B White, and P Swietach. 2024. “Protocol for Separating Cancer Cell Subpopulations by Metabolic Activity Using Flow Cytometry.” STAR Protocols 5 (2).
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Files:: Blaszczak_et_al_2024_Protocol_for_separating.pdf

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Publisher copy:: 10.1016/j.xpro.2024.103105

Authors

+ Blaszczak, W More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Oncology
Sub department:: Oncology
Role:: Author

+ White, B More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Physiology Anatomy & Genetics
Role:: Author

+ Swietach, P More by this author

Institution:: University of Oxford
Division:: MSD
Department:: Physiology Anatomy & Genetics
Role:: Author
ORCID:: 0000-0002-9945-9473

Publisher:: Cell Press
Journal:: STAR Protocols More from this journal
Volume:: 5
Issue:: 2
Article number:: 103105
Publication date:: 2024-06-01
Acceptance date:: 2024-05-14
DOI:: 10.1016/j.xpro.2024.103105
EISSN:: 2666-1667

Language:: English
Pubs id:: 1996809
Local pid:: pubs:1996809
Deposit date:: 2024-05-16

Terms of use

Copyright holder:: Blaszczak et al.
Copyright date:: 2024
Rights statement:: © 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Licence:: CC Attribution (CC BY)

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