Journal article
High-resolution crystal structure of arthropod Eiger TNF suggests a mode of receptor engagement and altered surface charge within endosomes
- Abstract:
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The tumour necrosis factor alpha (TNFα) superfamily of proteins are critical in numerous biological processes, such as in development and immunity. Eiger is the sole TNFα member described in arthropods such as in the important model organism Drosophila. To date there are no structural data on any Eiger protein. Here we present the structure of the TNF domain of Eiger from the fall armyworm Spodoptera frugiperda (SfEiger) to 1.7 Å from a serendipitously obtained crystal without prior knowledge of the protein sequence. Our structure confirms that canonical trimerization is conserved from ancestral TNFs and points towards a mode of receptor engagement. Furthermore, we observe numerous surface histidines on SfEiger, potentially acting as pH switches following internalization into endosomes. Our data contributes to the genome annotation of S. frugiperda, a voracious agricultural pest, and can serve as a basis for future structure-function investigations of the TNF system in related arthropods such as Drosophila.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 2.8MB, Terms of use)
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- Publisher copy:
- 10.1038/s42003-019-0541-0
Authors
- Publisher:
- Nature Research
- Journal:
- Communications biology More from this journal
- Volume:
- 2
- Article number:
- 293
- Publication date:
- 2019-08-06
- Acceptance date:
- 2019-07-15
- DOI:
- EISSN:
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2399-3642
- Pmid:
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31396573
- Language:
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English
- Keywords:
- Pubs id:
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pubs:1041809
- UUID:
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uuid:9b3b28b6-7389-42cc-a89f-91cc98f2a41c
- Local pid:
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pubs:1041809
- Source identifiers:
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1041809
- Deposit date:
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2019-09-11
Terms of use
- Copyright holder:
- Bertinelli et al
- Copyright date:
- 2019
- Notes:
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© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative
Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
- Licence:
- CC Attribution (CC BY)
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