Journal article : Review
Proteases of Nicotiana benthamiana: an emerging battle for molecular farming.
- Abstract:
- Molecular farming increasingly uses the tobacco relative Nicotiana benthamiana for production of recombinant proteins through transient expression. Several proteins are produced efficiently with this expression platform, but yields for other proteins are often very low. These low yields are frequently due to endogenous proteases. The latest genome annotations indicate that N. benthamiana encodes for at least 1243 putative proteases that probably act redundantly and consecutively on substrates in different subcellular compartments. Here, we discuss the N. benthamiana protease repertoire that may affect recombinant protein production and recent advances in protease depletion strategies to increase recombinant protein production in N. benthamiana.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Accepted manuscript, 514.8KB, Terms of use)
-
- Publisher copy:
- 10.1016/j.copbio.2019.10.006
Authors
- Publisher:
- Elsevier
- Journal:
- Current Opinion in Biotechnology More from this journal
- Volume:
- 61
- Pages:
- 60-65
- Publication date:
- 2019-11-22
- Acceptance date:
- 2019-10-17
- DOI:
- EISSN:
-
1879-0429
- ISSN:
-
0958-1669
- Pmid:
-
31765962
- Language:
-
English
- Keywords:
- Subtype:
-
Review
- Pubs id:
-
1076299
- Local pid:
-
pubs:1076299
- Deposit date:
-
2020-03-30
Terms of use
- Copyright holder:
- Crown Copyright
- Copyright date:
- 2019
- Rights statement:
- Crown Copyright © 2019 Published by Elsevier Ltd. All rights reserved.
- Notes:
-
This is the accepted manuscript version of the article. The final version is available from Elsevier at https://doi.org/10.1016/j.copbio.2019.10.006
If you are the owner of this record, you can report an update to it here: Report update to this record