Journal article
Pleural fluid has pro-growth biological properties which enable cancer cell proliferation
- Abstract:
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Objectives: Patients with malignant pleural mesothelioma (MPM) or pleural metastases often present with malignant pleural effusion (MPE). This study aimed to analyze the effect of pleural fluid on cancer cells.
Materials and Methods: Established patient-derived cancer cell cultures derived from MPE (MPM, breast carcinoma, lung adenocarcinoma) were seeded in 100% pleural fluid (exudate MPM MPE, transudate MPE, non-MPE transudate fluid) and proliferation was monitored. In addition, the establishment of new MPM cell cultures, derived from MPE specimens, was attempted by seeding the cells in 100% MPE fluid.
Results: All established cancer cell cultures proliferated with similar growth rates in the different types of pleural fluid. Primary MPM cell culture success was similar with MPE fluid as with full culture medium.
Conclusions: Pleural fluid alone is adequate for cancer cell proliferation in vitro, regardless of the source of pleural fluid. These results support the hypothesis that pleural fluid has important pro-growth biological properties, but the mechanisms for this effect are unclear and likely not malignant effusion specific.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, 5.6MB, Terms of use)
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- Publisher copy:
- 10.3389/fonc.2021.658395
Authors
- Publisher:
- Frontiers Media
- Journal:
- Frontiers in Oncology More from this journal
- Volume:
- 11
- Article number:
- 658395
- Place of publication:
- Switzerland
- Publication date:
- 2021-04-28
- Acceptance date:
- 2021-03-15
- DOI:
- EISSN:
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2234-943X
- Pmid:
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33996582
- Language:
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English
- Keywords:
- Pubs id:
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1177355
- Local pid:
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pubs:1177355
- Deposit date:
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2022-02-12
Terms of use
- Copyright holder:
- Asciak et al.
- Copyright date:
- 2021
- Rights statement:
- ©2021 Asciak, Kanellakis, Yao, Abd Hamid, Mercer, Hassan, Bedawi, Dobson, Fsadni, Montefort, Dong, Rahman and Psallidas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Licence:
- CC Attribution (CC BY)
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