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Metabolomics of 293T IDH1 mutant and wild-type cells

Documentation:
These data were produced from the analysis of the 293T cells with (MUT) and without (WT) a transduced isocitrate dehydrogenase 1 (IDH1) mutation. The data were acquired as part of the project “Investigating Isocitrate Dehydrogenase Mutations in Cancer Using New Metabolomics Methods” using multiple liquid chromatography-mass spectrometry methods. Anion-exchange chromatography-mass spectrometry (IC-MS) data were acquired on a Dionex IC-5000+ ion chromatography system hyphenated to a Thermo Fisher Scientific Exactive. Reversed-phase chromatography-mass spectrometry (RP-MS) data were acquired on a Dionex U3000 UHPLC system hyphenated to a Thermo Fisher Scientific Exactive. Derivatised RP-MS data were acquired on a Waters ACQUITY UPLC System hyphenated to a Waters Xevo G2-XS QTof with sample derivatisation using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate. Data are .zip compressed to reduce file size. Data files in the .RAW format require Thermo Fisher Scientific Xcalibur software to analyse. Data folders in the .RAW format require Waters Masslynx software to analyse.

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Role:
Creator

Contributors

Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Role:
Principal Investigator (PI)
ORCID:
0000-0003-4733-1205

Publisher:
University of Oxford
Publication date:
2020
DOI:

Language:
English
Keywords:
Subjects:
Deposit date:
2020-06-18

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