Journal article
A new paradigm for outer membrane protein biogenesis in the Bacteroidota
- Abstract:
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In Gram-negative bacteria, the outer membrane is the first line of defence against antimicrobial agents and immunological attacks. A key part of outer membrane biogenesis is the insertion of outer membrane proteins by the β-barrel-assembly machinery (BAM). Here we report the cryo-electron microscopy structure of a BAM complex isolated from Flavobacterium johnsoniae, a member of the Bacteroidota, a phylum that includes key human commensals and major anaerobic pathogens. This BAM complex is extensively modified from the canonical Escherichia coli system and includes an extracellular canopy that overhangs the substrate folding site and a subunit that inserts into the BAM pore. The novel BamG and BamH subunits that are involved in forming the extracellular canopy are required for BAM function and are conserved across the Bacteroidota, suggesting that they form an essential extension to the canonical BAM core in this phylum. For BamH, isolation of a suppressor mutation enables the separation of its essential and non-essential functions. The need for a highly remodelled and enhanced BAM complex reflects the unusually complex membrane proteins found in the outer membrane of the Bacteroidota.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 39.6MB, Terms of use)
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- Publisher copy:
- 10.1038/s41586-025-09532-8
Authors
- Funder identifier:
- https://ror.org/0472cxd90
- Grant:
- 833713
- Publisher:
- Springer Nature
- Journal:
- Nature More from this journal
- Volume:
- 647
- Issue:
- 8089
- Pages:
- 479–487
- Publication date:
- 2025-10-01
- Acceptance date:
- 2025-08-14
- DOI:
- EISSN:
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1476-4687
- ISSN:
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0028-0836
- Language:
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English
- Pubs id:
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2280716
- Local pid:
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pubs:2280716
- Deposit date:
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2025-08-14
- ARK identifier:
Terms of use
- Copyright holder:
- Liu et al.
- Copyright date:
- 2025
- Rights statement:
- © The Author(s) 2025. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
- Licence:
- CC Attribution (CC BY)
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