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Probing the penicillin sidechain selectivity of recombinant deacetoxycephalosporin C synthase.

Abstract:

Deacetoxycephalosporin C synthase from Streptomyces clavuligerus catalyses the conversion of the five-membered penicillin ring to the unsaturated six-membered cephem ring of deacetoxycephalosporin C. The effects on enzyme activity of the penicillin substrate sidechain and various cofactors were investigated using a continuous spectrophotometric assay. The conversion of penicillin G to phenylacetyl-7-aminodeacetoxycephalo sporanic acid (G-7-ADCA) was confirmed, and further details of the react...

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Publication status:
Published

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Publisher copy:
10.1007/pl00000904

Authors


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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Baldwin, JE More by this author
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Journal:
Cellular and molecular life sciences : CMLS
Volume:
58
Issue:
5-6
Pages:
835-843
Publication date:
2001-05-05
DOI:
EISSN:
1420-9071
ISSN:
1420-682X
URN:
uuid:99e72f1e-d8a1-444f-8003-1c9e29ed0cf6
Source identifiers:
37839
Local pid:
pubs:37839

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