Journal article icon

Journal article

Evaluation of metalloendopeptidase Lys-N protease performance under different sample handling conditions.

Abstract:

Trypsin, the most widely used enzyme in proteomics, has a few caveats as it does not perform well under certain harsh sample handling conditions and creates relatively short peptides less amenable to, for instance, electron transfer dissociation. There is, thus, room for improvement using alternative proteases. Here, we evaluate the performance of such an alternative protease, the metalloendopeptidase Lys-N, in sample preparation for proteomic analyses under various experimental conditions. T...

Expand abstract
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1021/pr100341e

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Role:
Author
Journal:
Journal of proteome research
Volume:
9
Issue:
8
Pages:
4282-4288
Publication date:
2010-08-05
DOI:
EISSN:
1535-3907
ISSN:
1535-3893
URN:
uuid:98e0c16d-2210-4228-9406-2bd8e66c6516
Source identifiers:
384582
Local pid:
pubs:384582

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP