Journal article
Evaluation of metalloendopeptidase Lys-N protease performance under different sample handling conditions.
- Abstract:
-
Trypsin, the most widely used enzyme in proteomics, has a few caveats as it does not perform well under certain harsh sample handling conditions and creates relatively short peptides less amenable to, for instance, electron transfer dissociation. There is, thus, room for improvement using alternative proteases. Here, we evaluate the performance of such an alternative protease, the metalloendopeptidase Lys-N, in sample preparation for proteomic analyses under various experimental conditions. T...
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- Publication status:
- Published
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- Publisher copy:
- 10.1021/pr100341e
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Authors
Bibliographic Details
- Journal:
- Journal of proteome research
- Volume:
- 9
- Issue:
- 8
- Pages:
- 4282-4288
- Publication date:
- 2010-08-01
- DOI:
- EISSN:
-
1535-3907
- ISSN:
-
1535-3893
- Source identifiers:
-
384582
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:384582
- UUID:
-
uuid:98e0c16d-2210-4228-9406-2bd8e66c6516
- Local pid:
- pubs:384582
- Deposit date:
- 2013-11-16
Terms of use
- Copyright date:
- 2010
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