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Evaluation of metalloendopeptidase Lys-N protease performance under different sample handling conditions.

Abstract:

Trypsin, the most widely used enzyme in proteomics, has a few caveats as it does not perform well under certain harsh sample handling conditions and creates relatively short peptides less amenable to, for instance, electron transfer dissociation. There is, thus, room for improvement using alternative proteases. Here, we evaluate the performance of such an alternative protease, the metalloendopeptidase Lys-N, in sample preparation for proteomic analyses under various experimental conditions. T...

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Publication status:
Published

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Publisher copy:
10.1021/pr100341e

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
Journal:
Journal of proteome research
Volume:
9
Issue:
8
Pages:
4282-4288
Publication date:
2010-08-01
DOI:
EISSN:
1535-3907
ISSN:
1535-3893
Source identifiers:
384582
Language:
English
Keywords:
Pubs id:
pubs:384582
UUID:
uuid:98e0c16d-2210-4228-9406-2bd8e66c6516
Local pid:
pubs:384582
Deposit date:
2013-11-16

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