Double T cell depletion of bone marrow using sequential positive and negative cell immunoaffinity or CD34+ cell selection followed by Campath-1M; effect on CD34+ cells and progenitor cell recoveries.

Clarke, E; Potter, M; Hale, G; Waldmann, H; Lankester, A; Cornish, J; Steward, C; Marks, D; Oakhill, A; Pamphilon, D

Journal article

Double T cell depletion of bone marrow using sequential positive and negative cell immunoaffinity or CD34+ cell selection followed by Campath-1M; effect on CD34+ cells and progenitor cell recoveries.

Abstract:: An advantage of CD34+ cell selection over antibody purging is that a component allograft is produced comprising a stem cell enriched and an unadsorbed fraction, the latter containing T cells which may be used for post-transplant immunotherapy. Initial reports with PBSC allografts suggested that T cell depletion (TCD) by CD34+ cell selection and post-graft cyclosporin A +/- methotrexate was insufficient prophylaxis against acute GVHD. We compared sequential TCD (of a CD34+ cell-selected fraction) using a second (CD2) immunoaffinity step or Campath-1M monoclonal antibody and complement. Since a high stem cell 'dose' enhances engraftment across HLA barriers and improves overall post-transplant outcome, the recovery of CD34+ cells and progenitors were assessed. Sequential positive (CD34+) and negative (CD2+) immunoaffinity selection resulted in a 3.4 log depletion of T cells as compared to a 4.05 log depletion when CD34+ cell selection was followed by Campath-1M treatment. Recoveries of CD34+ cells, CFU-GM and BFU-E following double depletion using CD34+ cell selection plus CD2+ cell depletion were 28, 25 and 17% as compared to 20, 18 and 16% when CD34+ cells were treated with Campath-1M. The unadsorbed fraction contained 85% of the original T cells, from which donor leukocyte infusions in the range of 10(5) to 10(7) CD3+ cells per kg body weight of the recipient were harvested. Despite the advantages of component allografts, the loss of stem/progenitor cells may restrict sequential TCD steps unless single BM harvests are supplemented and/or replaced with mobilised PBSCs.

Publication status:: Published

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APA Style

Clarke, E., Potter, M., Hale, G., Waldmann, H., Lankester, A., Cornish, J., Steward, C., Marks, D., Oakhill, A., & Pamphilon, D. (1998). Double T cell depletion of bone marrow using sequential positive and negative cell immunoaffinity or CD34+ cell selection followed by Campath-1M; effect on CD34+ cells and progenitor cell recoveries. Bone Marrow Transplantation, 22(2), 117–124.

MLA Style

Clarke, E, et al. “Double T Cell Depletion of Bone Marrow Using Sequential Positive and Negative Cell Immunoaffinity or CD34+ Cell Selection Followed by Campath-1M; Effect on CD34+ Cells and Progenitor Cell Recoveries.” Bone Marrow Transplantation, vol. 22, no. 2, 1998, pp. 117–24.

Chicago Style

Clarke, E, M Potter, G Hale, et al. 1998. “Double T Cell Depletion of Bone Marrow Using Sequential Positive and Negative Cell Immunoaffinity or CD34+ Cell Selection Followed by Campath-1M; Effect on CD34+ Cells and Progenitor Cell Recoveries.” Bone Marrow Transplantation 22 (2): 117–24.
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