Journal article icon

Journal article

Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups.

Abstract:
A series of non-nucleoside-based 2,4-dinitrophenyl (DNP) phosphoramidites have been prepared and used in the multiple labelling of oligonucleotides during solid-phase synthesis. The length of spacer arm between the DNP label and the oligonucleotide phosphate backbone, and the number of attached DNP groups have both been varied in order to determine the optimum conditions for anti-DNP antibody binding. Detection using enzyme-linked colorimetric techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.
Publication status:
Published

Actions


Access Document


Publisher copy:
10.1093/nar/21.8.1705

Authors


More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Organic Chemistry
Role:
Author
Journal:
Nucleic acids research
Volume:
21
Issue:
8
Pages:
1705-1712
Publication date:
1993-04-01
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
Source identifiers:
400088
Language:
English
Keywords:
Pubs id:
pubs:400088
UUID:
uuid:97b32d31-d440-4260-afa7-0f2ae349abfe
Local pid:
pubs:400088
Deposit date:
2013-11-16

Terms of use


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP