Journal article
Multicenter validation of CSF neurofilaments as diagnostic biomarkers for ALS
- Abstract:
-
Objective:
Neurofilaments are leading neurochemical biomarkers for amyotrophic lateral sclerosis (ALS). Here, we investigated the effect of preanalytical factors on neurofilament concentrations in cerebrospinal fluid (CSF) in a “reverse” round-robin with 15 centers across Europe/U.S.
Methods:
Samples from ALS and control patients (5/5 each center, n = 150) were analyzed for phosphorylated neurofilament heavy chain (pNfH) and neurofilament light chain (NfL) at two laboratories.
Results:
CSF pNfH was increased (p < 0.05) in ALS in 10 out of 15 centers and NfL in 5 out of 12 centers. The coefficient of variation (CV%) of pNfH measurements between laboratories was 18.7 ± 19.1%. We calculated a diagnostic cut-off of >568.5 pg/mL for pNfH (sensitivity 78.7%, specificity 93.3%) and >1,431pg/mL for NfL (sensitivity 79.0%, specificity 86.4%).
Conclusion:
Values in ALS patients are already comparable between most centers, supporting eventual implementation into clinical routine. However, continuous quality control programs will be necessary for inclusion in the diagnostic work-up.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Accepted manuscript, pdf, 627.6KB, Terms of use)
-
- Publisher copy:
- 10.3109/21678421.2016.1167913
Authors
+ Medical Research Council
More from this funder
- Funder identifier:
- https://ror.org/03x94j517
- Funding agency for:
- Turner, MR
- Grant:
- MR/K01014X/1
+ European Commission
More from this funder
- Funder identifier:
- https://ror.org/00k4n6c32
- Grant:
- 01ED1202A
- Programme:
- SOPHIA
+ European Commission
More from this funder
- Funder identifier:
- https://ror.org/00k4n6c32
- Grant:
- 01ED1203F
- Programme:
- BiomarkAPD
+ Türkiye Bilimsel ve Teknolojik Araştırma Kurumu
More from this funder
- Funder identifier:
- https://ror.org/04w9kkr77
- Grant:
- TUBITAK 112S362
- Publisher:
- Taylor and Francis
- Journal:
- Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration More from this journal
- Volume:
- 17
- Issue:
- 5-6
- Pages:
- 403-413
- Publication date:
- 2016-04-11
- Acceptance date:
- 2016-02-12
- DOI:
- EISSN:
-
2167-9223
- ISSN:
-
2167-8421
- Language:
-
English
- Keywords:
- Pubs id:
-
pubs:617696
- UUID:
-
uuid:97983edf-8c2d-4d49-80a8-d3dd0eb3bb35
- Local pid:
-
pubs:617696
- Source identifiers:
-
617696
- Deposit date:
-
2016-04-28
- ARK identifier:
Terms of use
- Copyright holder:
- World Federation of Neurology on behalf of the Research Group on Motor Neuron Diseases
- Copyright date:
- 2016
- Rights statement:
- © 2016 World Federation of Neurology on behalf of the Research Group on Motor Neuron Diseases.
If you are the owner of this record, you can report an update to it here: Report update to this record