Development and application of a fluoride-detection-based fluorescence assay for γ-butyrobetaine hydroxylase.

Journal article

Fluoride assays for oxygenases: The 2-oxoglutarate-dependent oxygenase BBOX catalyses the final step in carnitine biosynthesis and is a medicinal chemistry target. We report that BBOX can hydroxylate fluorinated substrates analogues with subsequent release of a fluoride ion, thereby enabling an efficient fluorescence-based assay.

Authors: Rydzik, A; Leung, I; Kochan, G; Thalhammer, A; Oppermann, U

• Publication status: Published
• Journal: Chembiochem : a European journal of chemical biology
• Volume: 13
• Issue: 11
• Pages: 1559-1563
• Publication date: 2012-07-01
• DOI: 10.1002/cbic.201200256
• EISSN: 1439-7633
• ISSN: 1439-4227
• Language: English
• Keywords: Protons; Biocatalysis; Hydrocarbons, Fluorinated; Humans; Magnetic Resonance Spectroscopy; Fluorescence; Molecular Structure; gamma-Butyrobetaine Dioxygenase; Fluorides