Journal article
Analysis of extracellular vesicles using fluorescence nanoparticle tracking analysis
- Abstract:
- Fluorescence nanoparticle tracking analysis (fl-NTA) allows for accurate sizing, counting, and phenotyping of extracellular vesicles (EV). Here, we present two protocols for the analysis of EVs using fl-NTA, highlighting the potential pitfalls and challenges. The first protocol utilizes CellMask Orange™ (CMO) as a general membrane marker to label EVs derived from plasma. The second protocol describes the use of a Qdot-conjugated antibody to identify syncytiotrophoblast (STB)-derived EVs. “Standard” preparations of STB-derived EVs enriched for either microvesicles (STBMV) or exosomes (STBEX), containing a known amount of EV positive for the STB specific antigen placental alkaline phosphatase (PLAP), were also used to optimize fl-NTA camera settings.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Authors
- Publisher:
- Humana Press
- Journal:
- Extracellular Vesicles More from this journal
- Volume:
- 1660
- Pages:
- 153-173
- Series:
- Methods in Molecular Biology
- Publication date:
- 2017-08-22
- Acceptance date:
- 2017-08-22
- DOI:
- ISSN:
-
1940-6029
- ISBN:
- 9781493972517
- Keywords:
- Pubs id:
-
pubs:745100
- UUID:
-
uuid:962f12f3-efb7-457e-bffe-0591e0df9314
- Local pid:
-
pubs:745100
- Source identifiers:
-
745100
- Deposit date:
-
2017-11-20
Terms of use
- Copyright holder:
- Springer Science+Business Media LLC
- Copyright date:
- 2017
- Notes:
- Copyright © 2017 Springer Science+Business Media LLC.
If you are the owner of this record, you can report an update to it here: Report update to this record