Solubilization of receptors for the novel Ca2+-mobilizing messenger, nicotinic acid adenine dinucleotide phosphate.

Journal article

Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca(2+) mobilizing agent in a variety of broken and intact cell preparations. In sea urchin egg homogenates, NAADP releases Ca(2+) independently of inositol trisphosphate or ryanodine receptor activation. Little, however, is known concerning the molecular target for NAADP. Here we report for the first time solubilization of NAADP receptors from sea urchin egg homogenates. Supernatant fractions, prepared following Triton X-100 tr...

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Authors: Berridge, G; Dickinson, G; Parrington, J; Galione, A; Patel, S

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 277
• Issue: 46
• Pages: 43717-43723
• Publication date: 2002-11-01
• DOI: 10.1074/jbc.m203224200
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Dose-Response Relationship, Drug; Calcium; Sucrose; Sea Urchins; NADP; Inositol 1,4,5-Trisphosphate; Ryanodine Receptor Calcium Release Channel; Octoxynol; Electrophoresis, Polyacrylamide Gel; Centrifugation, Density Gradient; Chromatography, Gel; Ligands; Animals; Protein Binding; Time Factors