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Journal article

Characterising cerebrovascular reactivity and the pupillary light response-a comparative study

Abstract:

Introduction: Smooth muscle is integral to multiple autonomic systems, including cerebrovascular dynamics through vascular smooth muscle cells and in ocular muscle dynamics, by regulating pupil size. In the brain, smooth muscle function plays a role in cerebrovascular reactivity (CVR) that describes changes in blood vessel calibre in response to vasoactive stimuli. Similarly, pupil size regulation can be measured using the pupillary light response (PLR), the pupil’s reaction to changes in light levels. The primary aim of this study was to explore the interplay between cerebral blood flow and pupil dynamics, evaluated using CVR and PLR, respectively.

Methods: A total of 20 healthy adults took part in a CVR gas stimulus protocol and a light and dark flash PLR protocol. CVR was calculated as the blood flow velocity change in the middle cerebral artery, measured using transcranial Doppler ultrasound in response to a 5% increase in CO2. Multiple PLR metrics were evaluated with a clinical pupillometer.

Results: CVR and PLR metrics were all within the expected physiological ranges for healthy adults. Nine different PLR metrics, assessed through the light and dark flash protocols, were compared against CVR. A significant negative relationship was observed between the latency of the PLR in the dark flash protocol and CVR. No statistically significant relationships were found between CVR and other PLR metrics.

Conclusion: This is the first study to investigate the relationship between cerebral blood flow and pupil dynamics. A significant relationship between dark flash latency and CVR was observed. Future work includes evaluating these relationships using more robust CVR and PLR measurement techniques in a larger, more diverse cohort. Notably, more research is warranted into the PLR using a dark flash protocol and its connection to cerebrovascular function.

Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.3389/fphys.2024.1384113

Authors

More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Sub department:
Institute of Biomedical Engineering
Role:
Author
ORCID:
0000-0002-9416-2064
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Sub department:
Institute of Biomedical Engineering
Role:
Author
ORCID:
0000-0003-4658-9925
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Sub department:
Institute of Biomedical Engineering
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MPLS
Department:
Engineering Science
Oxford college:
St Hilda's College
Role:
Author
ORCID:
0000-0002-3034-8986


More from this funder
Funder identifier:
https://ror.org/0439y7842
Grant:
EP/S021507/1
More from this funder
Funder identifier:
https://ror.org/04v48nr57


Publisher:
Frontiers Media
Journal:
Frontiers in Physiology More from this journal
Volume:
15
Article number:
1384113
Place of publication:
Switzerland
Publication date:
2024-08-08
Acceptance date:
2024-07-29
DOI:
EISSN:
1664-042X
Pmid:
39175613


Language:
English
Keywords:
Pubs id:
2022625
Local pid:
pubs:2022625
Deposit date:
2024-09-30
ARK identifier:

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