Journal article
Profiling gut microbiota and bile acid metabolism in critically ill children
- Abstract:
- Broad-spectrum antimicrobial use during the treatment of critical illness influences gastrointestinal fermentation endpoints, host immune response and metabolic activity including the conversion of primary to secondary bile acids. We previously observed reduced fermentation capacity in the faecal microbiota of critically ill children upon hospital admission. Here, we further explore the timecourse of the relationship between the microbiome and bile acid profile in faecal samples collected from critically ill children. The microbiome was assayed by sequencing of the 16S rRNA gene, and faecal water bile acids were measured by liquid chromatography mass spectrometry. In comparison to admission faecal samples, members of the Lachnospiraceae recovered during the late-acute phase (days 8-10) of hospitalisation. Patients with infections had a lower proportion of Lachnospiraceae in their gut microbiota than controls and patients with primary admitting diagnoses. Keystone species linked to ecological recovery were observed to decline with the length of PICU admission. These species were further suppressed in patients with systemic infection, respiratory failure, and undergoing surgery. Bile acid composition recovers quickly after intervention for critical illness which may be aided by the compositional shift in Lachnospiraceae. Our findings suggest gut microbiota recovery can be readily assessed via measurement of faecal bile acids.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.2MB, Terms of use)
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- Publisher copy:
- 10.1038/s41598-022-13640-0
Authors
+ National Institute for Health Research
More from this funder
- Funder identifier:
- https://ror.org/0187kwz08
- Grant:
- BRC-1215-20014
- Publisher:
- Springer Nature
- Journal:
- Scientific Reports More from this journal
- Volume:
- 12
- Issue:
- 1
- Article number:
- 10432
- Place of publication:
- England
- Publication date:
- 2022-06-21
- Acceptance date:
- 2022-05-26
- DOI:
- EISSN:
-
2045-2322
- Pmid:
-
35729169
- Language:
-
English
- Pubs id:
-
1267407
- Local pid:
-
pubs:1267407
- Deposit date:
-
2025-01-14
- ARK identifier:
Terms of use
- Copyright holder:
- Kean et al
- Copyright date:
- 2022
- Rights statement:
- ©2022 The Authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
- Licence:
- CC Attribution (CC BY)
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