Differential regulation of extracellular tissue inhibitor of metalloproteinases-3 levels by cell membrane-bound and shed low density lipoprotein receptor-related protein 1.

Journal article

Tissue inhibitor of metalloproteinases-3 (TIMP-3) plays a key role in regulating extracellular matrix turnover by inhibiting matrix metalloproteinases (MMPs), adamalysins (ADAMs), and adamalysins with thrombospondin motifs (ADAMTSs). We demonstrate that levels of this physiologically important inhibitor can be regulated post-translationally by endocytosis. TIMP-3 was endocytosed and degraded by a number of cell types including chondrocytes, fibroblasts, and monocytes, and we found that the en...

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Authors: Scilabra, S; Troeberg, L; Yamamoto, K; Emonard, H; Thøgersen, I

• Publication status: Published
• Journal: Journal of biological chemistry
• Volume: 288
• Issue: 1
• Pages: 332-342
• Publication date: 2013-01-01
• DOI: 10.1074/jbc.m112.393322
• EISSN: 1083-351X
• ISSN: 0021-9258
• Language: English
• Keywords: Models, Biological; Humans; Tissue Inhibitor of Metalloproteinase-3; Phenotype; Enzyme-Linked Immunosorbent Assay; CHO Cells; Cricetinae; Cell Line, Tumor; Endocytosis; Microscopy, Confocal; Syndecan-1; Low Density Lipoprotein Receptor-Related Protein-1; Animals; Gene Expression Regulation, Enzymologic; Cell Membrane; Heparitin Sulfate