Journal article
Profiling of naïve and primed human pluripotent stem cells reveals state-associated miRNAs
- Abstract:
- Naïve human pluripotent stem cells (hPSC) resemble the embryonic epiblast at an earlier time-point in development than conventional, ‘primed’ hPSC. We present a comprehensive miRNA profiling of naïve-to-primed transition in hPSC, a process recapitulating aspects of early in vivo embryogenesis. We identify miR-143-3p and miR-22-3p as markers of the naïve state and miR-363-5p, several members of the miR-17 family, miR-302 family as primed markers. We uncover that miR-371-373 are highly expressed in naïve hPSC. MiR-371-373 are the human homologs of the mouse miR-290 family, which are the most highly expressed miRNAs in naïve mouse PSC. This aligns with the consensus that naïve hPSC resemble mouse naive PSC, showing that the absence of miR-371-373 in conventional hPSC is due to cell state rather than a species difference.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, 1.9MB, Terms of use)
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- Publisher copy:
- 10.1038/s41598-020-67376-w
Authors
- Publisher:
- Nature Research
- Journal:
- Scientific Reports More from this journal
- Volume:
- 10
- Article number:
- 10542
- Place of publication:
- England
- Publication date:
- 2020-06-29
- Acceptance date:
- 2020-06-08
- DOI:
- EISSN:
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2045-2322
- Pmid:
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32601281
- Language:
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English
- Keywords:
- Pubs id:
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1115582
- Local pid:
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pubs:1115582
- Deposit date:
-
2020-08-20
Terms of use
- Copyright holder:
- Dodsworth et al.
- Copyright date:
- 2020
- Rights statement:
- Copyright © 2020 The Author(s). Licence to Publish: (licence type: CC-BY, government type: NONE), completed by Sally (A) Cowley on 8th June 20 Techniques: Life sciences techniques [Cell/tissue technologies]; Life sciences techniques, Cell/tissue technologies [Stem cells]; Life sciences techniques [Gene expression analysis]; Life sciences techniques [Genomic analysis]; Life sciences techniques, Genomic analysis [RNA isolation and purification]; Life sciences techniques, Genomic analysis [RNA sequencing]; CTS received date: 08.06.2020.
- Licence:
- CC Attribution (CC BY)
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