Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis.

Journal article

Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

Authors: Chantratita, N; Wuthiekanun, V; Thanwisai, A; Limmathurotsakul, D; Cheng, A

• Publication status: Published
• Journal: Clinical and vaccine immunology : CVI
• Volume: 14
• Issue: 1
• Pages: 110-113
• Publication date: 2007-01-01
• DOI: 10.1128/cvi.00289-06
• EISSN: 1556-679X
• ISSN: 1556-6811
• Language: English
• Keywords: Antigens, Bacterial; Melioidosis; Antibodies, Bacterial; Burkholderia pseudomallei; Sensitivity and Specificity; Enzyme-Linked Immunosorbent Assay; Hemagglutination Inhibition Tests; Serologic Tests