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Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis.

Abstract:
Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).
Publication status:
Published

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Publisher copy:
10.1128/cvi.00289-06

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Thailand/Laos MOP
Wuthiekanun, V More by this author
Thanwisai, A More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Clinical Medicine, Thailand/Laos MOP
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Journal:
Clinical and vaccine immunology : CVI
Volume:
14
Issue:
1
Pages:
110-113
Publication date:
2007-01-05
DOI:
EISSN:
1556-679X
ISSN:
1556-6811
URN:
uuid:93b93a17-4ee2-4d35-a134-04188669e8f6
Source identifiers:
30117
Local pid:
pubs:30117

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