Journal article
Post-Ganglionic sympathetic neurons can directly sense raised extracellular Na+ via SCN7a/Nax
- Abstract:
- The relationship between dietary NaCl intake and high blood pressure is well-established, and occurs primarily through activation of the sympathetic nervous system. Nax, a Na+-sensitive Na+ channel, plays a pivotal role in driving sympathetic excitability, which is thought to originate from central regions controlling neural outflow. We investigated whether post-ganglionic sympathetic neurons from different ganglia innervating cardiac and vasculature tissue can also directly sense extracellular Na+. Using whole-cell patch clamp recordings we demonstrate that sympathetic neurons from three sympathetic ganglia (superior cervical, stellate and superior mesenteric/coeliac) respond to elevated extracellular NaCl concentration. In sympathetic stellate ganglia neurons, we established that the effect of NaCl was dose-dependent and independent of osmolarity, Cl− and membrane Ca2+ flux, and critically dependent on extracellular Na+ concentration. We show that Nax is expressed in sympathetic stellate ganglia neurons at a transcript and protein level using single-cell RNA-sequencing and immunohistochemistry respectively. Additionally, the response to NaCl was prevented by siRNA-mediated knockdown of Nax, but not by inhibition of other membrane Na+ pathways. Together, these results demonstrate that post-ganglionic sympathetic neurons are direct sensors of extracellular Na+ via Nax, which could contribute to sympathetic driven hypertension.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, 2.3MB, Terms of use)
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- Publisher copy:
- 10.3389/fphys.2022.931094
Authors
- Publisher:
- Frontiers Media
- Journal:
- Frontiers in Physiology More from this journal
- Volume:
- 13
- Article number:
- 931094
- Publication date:
- 2022-06-17
- Acceptance date:
- 2022-05-24
- DOI:
- EISSN:
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1664-042X
- Pmid:
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35784866
- Language:
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English
- Keywords:
- Pubs id:
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1267116
- Local pid:
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pubs:1267116
- Deposit date:
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2022-07-13
Terms of use
- Copyright holder:
- Davis et al.
- Copyright date:
- 2022
- Rights statement:
- ©2022 Davis, Paterson and Herring. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Licence:
- CC Attribution (CC BY)
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