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PROTOPLAST FORMATION AND REGENERATION FROM SPORANGIA AND ENCYSTED ZOOSPORES OF PHYTOPHTHORA-INFESTANS

Abstract:

Protoplasts of Phytophthora infestans were formed from sporangia or encysted zoospores by digestion with NovoZym 234 in stabilizing osmotica. The highest yields, representing a conversion of up to 90% of initial sporangia or cysts were obtained in solKCl CaCl2 mixtures with osmolalities around 1·7 osmolals. Protoplast integrity was determined by autolysis in water, absence of fluorescence after Calcofluor treatment and protoplast fusion after treatment with polyethylene glycol. Protoplasts fo...

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Publication status:
Published

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Authors


CAMPBELL, A More by this author
KINGHORN, J More by this author
Journal:
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
Volume:
34
Issue:
4
Pages:
299-307
Publication date:
1989-04-05
DOI:
EISSN:
1096-1178
ISSN:
0885-5765
URN:
uuid:91802e5d-c3ca-4f0e-98cd-c08a2ab9e70e
Source identifiers:
43073
Local pid:
pubs:43073
Language:
English

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