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A modified chromatin-immunoprecipitation protocol for silkmoth ovarian follicular cells reveals C/EBP and GATA binding modes on an early chorion gene promoter.

Abstract:

Standard Chromatin immunoprecipitation protocols have been designed to suit studies performed on cell line cultures or yeast cells growing in liquid cultures. In these cases cross-linking/fixation takes place directly in the growing medium of the cells by the addition of a general fixation reagent. When applied on whole isolated silkmoth follicles, this procedure results in poor release of follicular cells from the basal membrane and lower yield of cross-linked chromatin. We present a modific...

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Publication status:
Published

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Publisher copy:
10.1007/s11033-008-9236-1

Authors


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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Lecanidou, R More by this author
Journal:
Molecular biology reports
Volume:
36
Issue:
4
Pages:
733-736
Publication date:
2009-04-05
DOI:
EISSN:
1573-4978
ISSN:
0301-4851
URN:
uuid:91789dc2-be44-420a-b058-0b5302446bf6
Source identifiers:
56313
Local pid:
pubs:56313

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