Islet transplantation into brown adipose tissue can delay immune rejection

Kepple, JD; Barra, JM; Young, ME; Hunter, CS; Tse, HM

Journal article

Islet transplantation into brown adipose tissue can delay immune rejection

Abstract:: Type 1 diabetes is an autoimmune disease characterized by insulin-producing β cell destruction. Although islet transplantation restores euglycemia and improves patient outcomes, an ideal transplant site remains elusive. Brown adipose tissue (BAT) has a highly vascularized and antiinflammatory microenvironment. Because these tissue features can promote islet graft survival, we hypothesized that islets transplanted into BAT will maintain islet graft and BAT function while delaying immune-mediated rejection. We transplanted syngeneic and allogeneic islets into BAT or under the kidney capsule of streptozotocin-induced diabetic NOD.Rag and NOD mice to investigate islet graft function, BAT function, metabolism, and immune-mediated rejection. Islet grafts within BAT restored euglycemia similarly to kidney capsule controls. Islets transplanted in BAT maintained expression of islet hormones and transcription factors and were vascularized. Compared with those in kidney capsule and euglycemic mock-surgery controls, no differences in glucose or insulin tolerance, thermogenic regulation, or energy expenditure were observed with islet grafts in BAT. Immune profiling of BAT revealed enriched antiinflammatory macrophages and T cells. Compared with the kidney capsule control, there were significant delays in autoimmune and allograft rejection of islets transplanted in BAT, possibly due to increased antiinflammatory immune populations. Our data support BAT as an alternative islet transplant site that may improve graft survival.

Publication status:: Published

Peer review status:: Peer reviewed

Actions

Email

Email this record

Send the bibliographic details of this record to your email address.

Your Email
Please enter the email address that the record information will be sent to.

-
Your message (optional)
Please add any additional information to be included within the email.
Share
Cite

Cite this record

APA Style

Kepple, J. D., Barra, J. M., Young, M. E., Hunter, C. S., & Tse, H. M. (2022). Islet transplantation into brown adipose tissue can delay immune rejection. JCI Insight, 7(4), e152800.

MLA Style

Kepple, JD, et al. “Islet Transplantation into Brown Adipose Tissue Can Delay Immune Rejection.” JCI Insight, vol. 7, no. 4, 2022, p. e152800.

Chicago Style

Kepple, JD, JM Barra, ME Young, CS Hunter, and HM Tse. 2022. “Islet Transplantation into Brown Adipose Tissue Can Delay Immune Rejection.” JCI Insight 7 (4): e152800.
Print