Journal article
Tertiary lymphoid structures critical for prognosis in endometrial cancer patients
- Abstract:
- B-cells play a key role in cancer suppression, particularly when aggregated in tertiary lymphoid structures (TLS). Here, we investigate the role of B-cells and TLS in endometrial cancer (EC). Single cell RNA-sequencing of B-cells shows presence of naive B-cells, cycling/germinal center B-cells and antibody-secreting cells. Differential gene expression analysis shows association of TLS with L1CAM overexpression. Immunohistochemistry and co-immunofluorescence show L1CAM expression in mature TLS, independent of L1CAM expression in the tumor. Using L1CAM as a marker, 378 of the 411 molecularly classified ECs from the PORTEC-3 biobank are evaluated, TLS are found in 19%. L1CAM expressing TLS are most common in mismatch-repair deficient (29/127, 23%) and polymerase-epsilon mutant EC (24/47, 51%). Multivariable Cox regression analysis shows strong favorable prognostic impact of TLS, independent of clinicopathological and molecular factors. Our data suggests a pivotal role of TLS in outcome of EC patients, and establishes L1CAM as a simple biomarker.Tertiary lymphoid structures (TLS) are associated with a reduced risk of cancer recurrence and improved response to immune checkpoint blockade in several tumor types. Here the authors identify L1CAM as a marker for mature TLS and show that the presence of TLS is associated with favorable prognosis in patients with endometrial cancer from the PORTEC-3 trial.Biological, physical and clinical aspects of cancer treatment with ionising radiatio
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 2.1MB, Terms of use)
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- Publisher copy:
- 10.1038/s41467-022-29040-x
Authors
+ Promedica Stiftung
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- Funder identifier:
- 10.13039/501100008307
- Grant:
- F-87701-41-01
+ Cancer Research UK
More from this funder
- Funder identifier:
- 10.13039/501100000289
- Grant:
- C26642/A27963
+ KWF Kankerbestrijding
More from this funder
- Funder identifier:
- 10.13039/501100004622
- Grant:
- KWF-2021-13400
- Publisher:
- Nature Research
- Journal:
- Nature Communications More from this journal
- Volume:
- 13
- Issue:
- 1
- Pages:
- 1373-1373
- Article number:
- 1373
- Publication date:
- 2022-03-16
- DOI:
- EISSN:
-
2041-1723
- ISSN:
-
2041-1723
- Language:
-
English
- Keywords:
- Pubs id:
-
1243149
- Local pid:
-
pubs:1243149
- Source identifiers:
-
W4220818710
- Deposit date:
-
2026-04-10
- ARK identifier:
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Terms of use
- Copyright date:
- 2022
- Licence:
- CC Attribution (CC BY)
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