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A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline transport systems.

Abstract:

We have worked out a simplification of the procedure described by Schmitz et al. (Biochim. Biophys. Acta (1973) 323, 98--112) for the preparation of brush border membranes from small intestine. The procedure ultimately adopted is simple, rapid, does not necessarily require scraping and can be started from fresh or frozen material. It can be scaled up easily, allowing a quick production of large amounts of brush border membrane vesicles. These vesicles prove to be excellently suited for transp...

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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
Journal:
Biochimica et biophysica acta
Volume:
506
Issue:
1
Pages:
136-154
Publication date:
1978-01-01
DOI:
ISSN:
0006-3002
Source identifiers:
25298
Language:
English
Keywords:
Pubs id:
pubs:25298
UUID:
uuid:90e15f5b-9acb-4bd3-8dd6-a1364b6af10f
Local pid:
pubs:25298
Deposit date:
2012-12-19

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