Journal article
Plasmodium vivax rhomboid-like protease 1 gene diversity in Thailand.
- Abstract:
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Background
Plasmodium vivax infection remains a major public health problem, especially along the Thailand border regions. We examined the genetic diversity of this parasite by analyzing singlenucleotide polymorphisms (SNPs) of the P. vivax rhomboid-like protease 1 gene (Pvrom1) in parasites collected from western (Tak province, Thai-Myanmar border) and eastern (Chanthaburi province, Thai-Cambodia border) regions.
Methods
Data were collected by a cross-sectional survey, consisting of 47 and 45 P. vivax-infected filter paper-spotted blood samples from the western and eastern regions of Thailand, respectively during September 2013 to May 2014. Extracted DNA was examined for presence of P. vivax using Plasmodium species-specific nested PCR. Pvrom1 gene was PCR amplified, sequenced and the SNP diversity was analyzed using F-STAT, DnaSP, MEGA and LIAN programs.
Results
Comparison of sequences of the 92 Pvrom1 831-base open reading frames with that of a reference sequence (GenBank acc. no. XM001615211) revealed 17 samples with a total of 8 polymorphic sites, consisting of singleton (exon 3, nt 645) and parsimony informative (exon 1, nt 22 and 39; exon 3, nt 336, 537 and 656; and exon 4, nt 719 and 748) sites, which resulted in six different deduced Pvrom1 variants. Non-synonymous to synonymous substitutions ratio estimated by the DnaSP program was 1.65 indicating positive selection, but the Z-tests of selection showed no significant deviations from neutrality for Pvrom1 samples from western region of Thailand. In addition McDonald Kreitman test (MK) showed not significant, and Fst values are not different between the two regions and the regions combined. Interestingly, only Pvrom1 exon 2 was the most conserved sequences among the four exons.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 711.8KB, Terms of use)
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- Publisher copy:
- 10.1016/j.exppara.2017.06.007
Authors
- Publisher:
- Elsevier
- Journal:
- Experimental Parasitology More from this journal
- Volume:
- 181
- Pages:
- 1-6
- Publication date:
- 2017-07-01
- Acceptance date:
- 2017-06-29
- DOI:
- EISSN:
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1090-2449
- ISSN:
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0014-4894
- Pmid:
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28690071
- Language:
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English
- Keywords:
- Pubs id:
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pubs:708015
- UUID:
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uuid:9085f6ce-c367-4c40-9210-1643078af94b
- Local pid:
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pubs:708015
- Source identifiers:
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708015
- Deposit date:
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2017-08-29
Terms of use
- Copyright holder:
- Mahidol University
- Copyright date:
- 2017
- Notes:
- © 2017 Mahidol University. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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