Journal article
Exogenous amino acids are essential for interleukin-7 induced CD8 T cell growth
- Abstract:
- IL-7 signalling is important in regulating both survival and cellular size (growth) of T cells. While glucose metabolism has previously been implicated in the mechanism of IL-7 induced survival and growth, the role of amino acids has not before been reported. Here, we show IL-7 dependent T cell survival does not require either exogenous glucose or amino acids. In contrast, maintenance of cell size and IL-7 induced growth were specifically dependent on amino acids. Furthermore, cellular amino acid uptake was implicated in the mechanism of IL-7 induced growth. Analysis of IL-7 regulated gene expression revealed that neutral and cationic amino acid transporters were specific transcriptional targets of IL-7 signalling. In contrast, none of the four glucose transporters expressed in T cells were modulated. Taken together, these data reveal for the first time the central importance of amino acid homeostasis for IL-7 regulated T cell growth.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 364.7KB, Terms of use)
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- Publisher copy:
- 10.1371/journal.pone.0033998
Authors
+ Medical Research Council
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- Funder identifier:
- https://ror.org/03x94j517
- Grant:
- MC_U117573801
- Publisher:
- Public Library of Science
- Journal:
- PLoS One More from this journal
- Volume:
- 7
- Issue:
- 4
- Article number:
- e33998
- Place of publication:
- United States
- Publication date:
- 2012-04-17
- Acceptance date:
- 2012-02-20
- DOI:
- EISSN:
-
1932-6203
- Pmid:
-
22529903
- Language:
-
English
- Pubs id:
-
348061
- Local pid:
-
pubs:348061
- Deposit date:
-
2024-07-17
Terms of use
- Copyright holder:
- Pearson et al
- Copyright date:
- 2012
- Rights statement:
- © 2012 Pearson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Notes:
- A correction to this article is available online from PLOS at: https://doi.org/10.1371/annotation/ee9c3e82-855f-42e0-8e57-83981bc1ba16
- Licence:
- CC Attribution (CC BY)
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