Multilocus sequence typing directly on DNA from clinical samples and a cultured isolate to investigate linked fatal pneumococcal disease in residents of a shelter for homeless men.

Journal article

Two apparently linked fatal cases of pneumococcal meningitis were investigated. Pneumolysin PCR performed on blood and cerebrospinal fluid was positive in a culture-negative case. A second case yielded Streptococcus pneumoniae from blood culture. Multilocus sequence typing performed on DNA extracted from case 1 (specimens) and case 2 (isolate) revealed identical sequence types (ST53) substantiating the link between them.

Authors: Birtles, A; McCarthy, N; Sheppard, C; Rutter, H; Guiver, M

• Journal: Journal of clinical microbiology
• Volume: 43
• Issue: 4
• Pages: 2004-2008
• Publication date: 2005-04-01
• DOI: 10.1128/jcm.43.4.2004-2008.2005
• EISSN: 1098-660X
• ISSN: 0095-1137
• Language: English
• Keywords: Streptolysins; Humans; Streptococcus pneumoniae; Cerebrospinal Fluid; Polymerase Chain Reaction; Adult; Homeless Persons; Meningitis, Pneumococcal; Bacterial Proteins; Fatal Outcome; Sequence Analysis, DNA; Blood; Bacterial Typing Techniques; Male