Use of synthetic peptides of influenza nucleoprotein to define epitopes recognized by class I-restricted cytotoxic T lymphocytes.

Journal article

The conserved epitopes of influenza nucleoprotein (NP) recognized by class I MHC-restricted CTL from CBA (H-2k) and C57BL/10 (H-2b) mice have been defined in vitro with synthetic peptides 50-63 and 365-379, respectively. Two Db-restricted clones were described that recognize different epitopes on peptide 365-379. Finally, the recognition of complete NP was shown to be approximately 200-fold less efficient than peptide in the cytotoxicity assay. These phenomena are closely related to results with class II-restricted T cells and they strengthen the hypothesis that influenza proteins are degraded in the infected cell before recognition by class I-restricted CTL.

Authors: Bastin, J; Rothbard, J; Davey, J; Jones, I; Townsend, A

• Publication status: Published
• Journal: Journal of experimental medicine
• Volume: 165
• Issue: 6
• Pages: 1508-1523
• Publication date: 1987-06-01
• DOI: 10.1084/jem.165.6.1508
• EISSN: 1540-9538
• ISSN: 0022-1007
• Language: English
• Keywords: Epitopes; Viral Proteins; Histocompatibility Antigens; Female; Cytotoxicity Tests, Immunologic; Mice, Inbred C57BL; T-Lymphocytes, Cytotoxic; Nucleoproteins; Amino Acid Sequence; Mice; Influenza A virus; Animals