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Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A

Abstract:
Chromothripsis, the chaotic shattering and repair of chromosomes, is common in cancer. Whether chromothripsis generates actionable therapeutic targets remains an open question. In a cohort of 64 patients in blast phase of a myeloproliferative neoplasm (BP-MPN), we describe recurrent amplification of a region of chromosome 21q (‘chr. 21amp’) in 25%, driven by chromothripsis in a third of these cases. We report that chr. 21amp BP-MPN has a particularly aggressive and treatment-resistant phenotype. DYRK1A, a serine threonine kinase, is the only gene in the 2.7-megabase minimally amplified region that showed both increased expression and chromatin accessibility compared with non-chr. 21amp BP-MPN controls. DYRK1A is a central node at the nexus of multiple cellular functions critical for BP-MPN development and is essential for BP-MPN cell proliferation in vitro and in vivo, and represents a druggable axis. Collectively, these findings define chr. 21amp as a prognostic biomarker in BP-MPN, and link chromothripsis to a therapeutic target.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1038/s41588-025-02190-6

Authors

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Institution:
University of Oxford
Division:
MSD
Department:
Radcliffe Department of Medicine
Oxford college:
New College
Role:
Author
ORCID:
0000-0002-4028-3733
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Radcliffe Department of Medicine
Role:
Author
More by this author
Institution:
University of Oxford
Division:
MSD
Department:
Radcliffe Department of Medicine
Role:
Author


More from this funder
Funder identifier:
https://ror.org/00xsz4758
Grant:
2021/JGF/005
Programme:
John Goldman Fellowship
More from this funder
Funder identifier:
https://ror.org/052gg0110
Grant:
101/517
131/030
Programme:
John Fell Fund
More from this funder
Funder identifier:
https://ror.org/029chgv08
Grant:
222800/Z/21/Z
203141/Z/16/Z
220586/Z/20/Z
More from this funder
Funder identifier:
https://ror.org/054225q67
Grant:
RCCSCF-May24/100001
CTRQQR-2021\100002
C67633/ A29034
C42639/A26988
More from this funder
Funder identifier:
https://ror.org/0187kwz08
Grant:
NIHR203308


Publisher:
Springer Nature
Journal:
Nature Genetics More from this journal
Volume:
57
Issue:
6
Pages:
1478–1492
Publication date:
2025-06-09
Acceptance date:
2025-04-08
DOI:
EISSN:
1546-1718
ISSN:
1061-4036


Language:
English
Pubs id:
2129078
Local pid:
pubs:2129078
Deposit date:
2025-06-10
ARK identifier:

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