Journal article
Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano
- Abstract:
-
Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, the lack of transgenesis methods considerably hampers their wider use. Here we report development of a transgenesis method for Macrostomum lignano, a basal flatworm with excellent regeneration capacity. We demonstrate that microinjection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation, frequen...
Expand abstract
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Authors
Funding
+ Biotechnology and Biological Sciences Research Council
More from this funder
Funding agency for:
Aboobaker, A
Grant:
BB/K007564/1
Bibliographic Details
- Publisher:
- Springer Nature Publisher's website
- Journal:
- Nature Communications Journal website
- Volume:
- 8
- Issue:
- 1
- Article number:
- 2120
- Publication date:
- 2017-12-14
- Acceptance date:
- 2017-11-12
- DOI:
- ISSN:
-
2041-1723
- Pmid:
-
29242515
Item Description
- Language:
- English
- Pubs id:
-
pubs:811413
- UUID:
-
uuid:8e3a7a22-e381-4e4a-8c1b-bddd13cc063d
- Local pid:
- pubs:811413
- Source identifiers:
-
811413
- Deposit date:
- 2018-01-08
Terms of use
- Copyright holder:
- Wudarski et al
- Copyright date:
- 2017
- Notes:
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
Metrics
If you are the owner of this record, you can report an update to it here: Report update to this record