Journal article
Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano
- Abstract:
- Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, the lack of transgenesis methods considerably hampers their wider use. Here we report development of a transgenesis method for Macrostomum lignano, a basal flatworm with excellent regeneration capacity. We demonstrate that microinjection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation, frequently results in random integration of the transgene in the genome and its stable transmission through the germline. To facilitate selection of promoter regions for transgenic reporters, we assembled and annotated the M. lignano genome, including genome-wide mapping of transcription start regions, and show its utility by generating multiple stable transgenic lines expressing fluorescent proteins under several tissue-specific promoters. The reported transgenesis method and annotated genome sequence will permit sophisticated genetic studies on stem cells and regeneration using M. lignano as a model organism.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
Actions
Access Document
- Files:
-
-
(Preview, Version of record, pdf, 1.9MB, Terms of use)
-
- Publisher copy:
- 10.1038/s41467-017-02214-8
Authors
+ Biotechnology and Biological Sciences Research Council
More from this funder
- Funding agency for:
- Aboobaker, A
- Grant:
- BB/K007564/1
- Publisher:
- Springer Nature
- Journal:
- Nature Communications More from this journal
- Volume:
- 8
- Issue:
- 1
- Article number:
- 2120
- Publication date:
- 2017-12-14
- Acceptance date:
- 2017-11-12
- DOI:
- ISSN:
-
2041-1723
- Pmid:
-
29242515
- Language:
-
English
- Pubs id:
-
pubs:811413
- UUID:
-
uuid:8e3a7a22-e381-4e4a-8c1b-bddd13cc063d
- Local pid:
-
pubs:811413
- Source identifiers:
-
811413
- Deposit date:
-
2018-01-08
Terms of use
- Copyright holder:
- Wudarski et al
- Copyright date:
- 2017
- Notes:
- This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Licence:
- CC Attribution (CC BY)
If you are the owner of this record, you can report an update to it here: Report update to this record