DNA polymerase theta purified from human cells is a high-fidelity enzyme.

Journal article

With the aim to identify unconventional DNA polymerases from human cells, we have set up a special assay to fractionate HeLa extracts based on the ability (i) to bypass DNA lesions, (ii) to be resistant to aphidicolin and an inhibitory antibody against pol alpha and (iii) to be non-responsive to proliferating cell nuclear antigen. After eight different chromatographic steps, an aphidicolin-resistant DNA polymerase activity was obtained that was able to utilize either undamaged or abasic sites...

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Authors: Maga, G; Shevelev, I; Ramadan, K; Spadari, S; Hübscher, U

• Publication status: Published
• Journal: Journal of molecular biology
• Volume: 319
• Issue: 2
• Pages: 359-369
• Publication date: 2002-05-01
• DOI: 10.1016/s0022-2836(02)00325-x
• EISSN: 1089-8638
• ISSN: 0022-2836
• Language: English
• Keywords: DNA Replication; Kinetics; DNA-Directed DNA Polymerase; Exodeoxyribonucleases; Mutagenesis; Thermodynamics; Templates, Genetic; Humans; DNA; Hela Cells