The CRAPome: a contaminant repository for affinity purification-mass spectrometry data.

Journal article

Affinity purification coupled with mass spectrometry (AP-MS) is a widely used approach for the identification of protein-protein interactions. However, for any given protein of interest, determining which of the identified polypeptides represent bona fide interactors versus those that are background contaminants (for example, proteins that interact with the solid-phase support, affinity reagent or epitope tag) is a challenging task. The standard approach is to identify nonspecific interaction...

Expand abstract

Authors: Mellacheruvu, D; Wright, Z; Couzens, A; Lambert, J; St-Denis, N

• Publication status: Published
• Journal: Nature methods
• Volume: 10
• Issue: 8
• Pages: 730-736
• Publication date: 2013-08-01
• DOI: 10.1038/nmeth.2557
• EISSN: 1548-7105
• ISSN: 1548-7091
• Language: English
• Keywords: Mass Spectrometry; Chromatography, Affinity; Protein Interaction Mapping; Proteomics; Databases, Factual; Proteins; Humans