Cloning the human lysozyme cDNA: inverted Alu repeat in the mRNA and in situ hybridization for macrophages and Paneth cells.

Journal article

Lysozyme is a major secretory product of human and rodent macrophages and a useful marker for myelomonocytic cells. Based on the known human lysozyme amino acid sequence, oligonucleotides were synthesized and used as probes to screen a phorbol 12-myristate 13-acetate-treated U937 cDNA library. A full-length human lysozyme cDNA clone, pHL-2, was obtained and characterized. Sequence analysis shows that human lysozyme, like chicken lysozyme, has an 18-amino-acid-long signal peptide, but unlike t...

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Authors: Chung, L; Keshav, S; Gordon, S

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 85
• Issue: 17
• Pages: 6227-6231
• Publication date: 1988-09-01
• DOI: 10.1073/pnas.85.17.6227
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: Epithelium; DNA; RNA, Messenger; Base Sequence; Molecular Sequence Data; Humans; Repetitive Sequences, Nucleic Acid; Muramidase; Cloning, Molecular; Genes; Nucleic Acid Hybridization; Macrophages; Amino Acid Sequence