Identification of a novel second-generation circulating recombinant form (CRF48_01B) in Malaysia: a descendant of the previously identified CRF33_01B.

Journal article

A molecular epidemiological investigation conducted among injecting drug users in eastern Peninsular Malaysia in 2007 identified a cluster of sequences (n = 3) located outside any known HIV-1 genotype. Analyses of near full-length nucleotide sequences of these strains from individuals with no recognizable linkage revealed that they have an identical subtype structure comprised of CRF01_AE and subtype B', distinct from any known circulating recombinant forms (CRFs). This novel CRF, designated CRF48_01B, is closely related to CRF33_01B, previously identified in Kuala Lumpur. Phylogenetic analysis of multiple CRF48_01B genome regions showed that CRF48_01B forms a monophyletic cluster within CRF33_01B, suggesting that this new recombinant is very likely a descendant of CRF33_01B. CRF48_01B thus represents one of the first examples of a "second-generation" CRF, generated by additional crossover with pre-existing CRFs. Corroborating these results, Bayesian molecular clock analyses indicated that CRF48_01B emerged in approximately 2001, approximately approximately 8 years after the emergence of CRF33_01B.

Authors: Li, Y; Tee, K; Liao, H; Hase, S; Uenishi, R

• Publication status: Published
• Journal: Journal of acquired immune deficiency syndromes (1999)
• Volume: 54
• Issue: 2
• Pages: 129-136
• Publication date: 2010-06-01
• DOI: 10.1097/qai.0b013e3181d82ce5
• EISSN: 1944-7884
• ISSN: 1525-4135
• Language: English
• Keywords: Time Factors; Humans; HIV Infections; Genotype; HIV-1; Malaysia; Middle Aged; Adult; Male; Molecular Sequence Data; Reassortant Viruses; Phylogeny