Journal article
A novel real time imaging platform to quantify macrophage phagocytosis.
- Abstract:
-
Phagocytosis of pathogens, apoptotic cells and debris is a key feature of macrophage function in host defense and tissue homeostasis. Quantification of macrophage phagocytosis in vitro has traditionally been technically challenging. Here we report the optimization and validation of the IncuCyte ZOOM® real time imaging platform for macrophage phagocytosis based on pHrodo® pathogen bioparticles, which only fluoresce when localized in the acidic environment of the phagolysosome. Image analysis a...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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Authors
Funding
EPA Research Fund
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Bibliographic Details
- Publisher:
- Elsevier Publisher's website
- Journal:
- Biochemical pharmacology Journal website
- Publication date:
- 2016-07-27
- Acceptance date:
- 2016-07-14
- DOI:
- EISSN:
-
1873-2968
- ISSN:
-
0006-2952
- Pmid:
-
27475716
Item Description
- Language:
- English
- Keywords:
- Pubs id:
-
pubs:638197
- UUID:
-
uuid:8d784f7b-bed6-452b-96ed-7746fe3b6a04
- Local pid:
- pubs:638197
- Deposit date:
- 2016-09-20
Terms of use
- Copyright holder:
- Kapellos et al
- Copyright date:
- 2016
- Notes:
- © 2016 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license.
- Licence:
- CC Attribution (CC BY)
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