Redox options in two-dimensional electrophoresis.

Journal article

Two-dimensional electrophoresis is usually run on fully reduced samples. Under these conditions even covalently bound oligomers are dissociated and individual polypeptide chains may be fully unfolded by both, urea and SDS, which maximizes the number of resolved components and allows their pI and M(r) to be most accurately evaluated. However, various electrophoretic protocols for protein structure investigation require a combination of steps under varying redox conditions. We review here some ...

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Authors: Wait, R; Begum, S; Brambilla, D; Carabelli, A; Conserva, F

• Publication status: Published
• Journal: Amino acids
• Volume: 28
• Issue: 3
• Pages: 239-272
• Publication date: 2005-05-01
• DOI: 10.1007/s00726-005-0175-z
• EISSN: 1438-2199
• ISSN: 0939-4451
• Language: English
• Keywords: Animals; Rats; Proteins; Electrophoresis, Gel, Two-Dimensional; Oxidation-Reduction; Humans; Cattle; Isoelectric Focusing; Mice