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Redox options in two-dimensional electrophoresis.

Abstract:

Two-dimensional electrophoresis is usually run on fully reduced samples. Under these conditions even covalently bound oligomers are dissociated and individual polypeptide chains may be fully unfolded by both, urea and SDS, which maximizes the number of resolved components and allows their pI and M(r) to be most accurately evaluated. However, various electrophoretic protocols for protein structure investigation require a combination of steps under varying redox conditions. We review here some ...

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Publication status:
Published

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Publisher copy:
10.1007/s00726-005-0175-z

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
NDORMS
Role:
Author
Journal:
Amino acids More from this journal
Volume:
28
Issue:
3
Pages:
239-272
Publication date:
2005-05-01
DOI:
EISSN:
1438-2199
ISSN:
0939-4451
Language:
English
Keywords:
Pubs id:
pubs:229378
UUID:
uuid:8d01b847-941f-40f3-bcbd-0b61695b8ab4
Local pid:
pubs:229378
Source identifiers:
229378
Deposit date:
2013-11-16

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