The VITAL assay: a versatile fluorometric technique for assessing CTL- and NKT-mediated cytotoxicity against multiple targets in vitro and in vivo.

Journal article

Assessment of cell-mediated toxicity has traditionally been achieved by measuring the specific activity of enriched effector cell populations against antigen-loaded target cells labeled with radioactive isotopes in vitro. Fluorometric techniques are viewed as a promising alternative to the use of radioactive isotopes for these analyses. Direct assessment of cytotoxicity in vivo can be achieved by monitoring survival of injected fluorescent targets relative to a differentially labeled internal...

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Authors: Hermans, I; Silk, J; Yang, J; Palmowski, M; Gileadi, U

• Publication status: Published
• Journal: Journal of immunological methods
• Volume: 285
• Issue: 1
• Pages: 25-40
• Publication date: 2004-02-01
• DOI: 10.1016/j.jim.2003.10.017
• EISSN: 1872-7905
• ISSN: 0022-1759
• Language: English
• Keywords: Cell Line; Jurkat Cells; T-Lymphocyte Subsets; Humans; Antigens; Mice, Transgenic; Immunization; Cytotoxicity Tests, Immunologic; Fluorometry; Flow Cytometry; T-Lymphocytes, Cytotoxic; Killer Cells, Natural; Mice; Fluorescent Dyes; Mice, Inbred C57BL; Mice, Knockout; Animals