Metabolic studies of a fructose-intolerant yeast by in vivo 31P-nuclear magnetic resonance spectroscopy.

Journal article

The metabolic effects of the administration of fructose to a yeast expressing the cDNA for rat liver ketohexokinase have been investigated by 31P-nuclear magnetic resonance spectroscopy. Cessation of growth suffered by the yeast on exposure to 5 and 25 mM-fructose was accompanied by a large accumulation of fructose 1-phosphate at the expense of cytoplasmic orthophosphate and nucleoside triphosphate. Shifts in resonances were consistent with a drop in cytoplasmic pH. Arresting growth with 1 mM...

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Authors: Doyle, T; Spickett, C; Donaldson, I; Ratcliffe, R

• Publication status: Published
• Journal: Yeast (Chichester, England)
• Volume: 9
• Issue: 8
• Pages: 867-873
• Publication date: 1993-08-01
• DOI: 10.1002/yea.320090807
• EISSN: 1097-0061
• ISSN: 0749-503X
• Language: English
• Keywords: Saccharomyces cerevisiae; Fructokinases; Magnetic Resonance Spectroscopy; Recombinant Proteins; Animals; Fructose; Transformation, Genetic; Fructose Intolerance; Rats; Phosphorus Isotopes