Direct simulation of the self-assembly of a small DNA origami

Journal article

By using oxDNA, a coarse-grained nucleotide-level model of DNA, we are able to directly simulate the self-assembly of a small 384-base-pair origami from single-stranded scaffold and staple strands in solution. In general, we see attachment of new staple strands occurring in parallel, but with cooperativity evident for the binding of the second domain of a staple if the adjacent junction is already partially formed. For a system with exactly one copy of each staple strand, we observe a complete assembly pathway in an intermediate temperature window; at low temperatures successful assembly is prevented by misbonding while at higher temperature the free-energy barriers to assembly become too large for assembly on our simulation time scales. For high-concentration systems involving a large staple strand excess, we never see complete assembly because there are invariably instances where two copies of the same staple both bind to the scaffold, creating a kinetic trap that prevents the complete binding of either staple. This mutual staple blocking could also lead to aggregates of partially formed origamis in real systems, and helps to rationalize certain successful origami design strategies.

Authors: Snodin, BEK; Romano, F; Rovigatti, L; Ouldridge, TE; Louis, AA

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: American Chemical Society
• Journal: ACS Nano
• Volume: 10
• Issue: 2
• Pages: 1724-1737
• Publication date: 2016-01-14
• Acceptance date: 2015-12-10
• DOI: 10.1021/acsnano.5b05865
• EISSN: 1936-086X
• ISSN: 1936-0851
• Language: English
• Keywords: simulation; self-assembly; DNA nanotechnology; coarse-grained modeling; DNA origami