APC mutations in sporadic colorectal tumors: A mutational "hotspot" and interdependence of the "two hits".

Journal article

Although APC mutations occur at a high frequency in colorectal cancers, few studies have performed a comprehensive analysis by screening the whole gene for mutations and assessing allelic loss. APC seems to act as a tumor-suppressor gene in a "nonclassical" fashion: data from familial adenomatous polyposis (FAP) show that the site of the germ-line mutation determines the type of "second hit" in FAP tumors, and simple protein inactivation is selected weakly, if at all. In this study, we screen...

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Authors: Rowan, A; Lamlum, H; Ilyas, M; Wheeler, J; Straub, J

• Publication status: Published
• Journal: Proceedings of the National Academy of Sciences of the United States of America
• Volume: 97
• Issue: 7
• Pages: 3352-3357
• Publication date: 2000-03-01
• DOI: 10.1073/pnas.97.7.3352
• EISSN: 1091-6490
• ISSN: 0027-8424
• Language: English
• Keywords: DNA Primers; Mutation; Humans; Colorectal Neoplasms; Cytoskeletal Proteins; beta Catenin; Loss of Heterozygosity; Tumor Cells, Cultured; Trans-Activators; Base Sequence; Genes, APC