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Characterization of the RNA-interference pathway as a tool for reverse genetic analysis in the nascent phototrophic endosymbiosis, Paramecium bursaria

Abstract:

Endosymbiosis was fundamental for the evolution of eukaryotic complexity. Endosymbiotic interactions can be dissected through forward- and reverse-genetic experiments, such as RNA-interference (RNAi). However, distinguishing small (s)RNA pathways in a eukaryote–eukaryote endosymbiotic interaction is challenging. Here, we investigate the repertoire of RNAi pathway protein-encoding genes in the model nascent endosymbiotic system, Paramecium bursaria–Chlorella spp. Using comparative genomics and...

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Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1098/rsos.210140

Authors


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Institution:
University of Oxford
Division:
MPLS
Department:
Zoology
Role:
Author
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Role:
Author
ORCID:
0000-0002-4607-2064
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Name:
Royal Society
Grant:
URF\R\191005
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Name:
European Commission
Grant:
819507
Publisher:
Royal Society
Journal:
Royal Society Open Science More from this journal
Volume:
8
Issue:
4
Article number:
210140
Publication date:
2021-04-21
Acceptance date:
2021-03-31
DOI:
EISSN:
2054-5703
Language:
English
Keywords:
Pubs id:
1174423
Local pid:
pubs:1174423
Deposit date:
2021-05-10

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