Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA processing

Journal article

In the nervous system, alternative RNA processing is particularly prevalent, which results in the expression of thousands of transcript variants found in no other tissue. Neuron-specific RNA-binding proteins co-transcriptionally regulate alternative splicing, alternative polyadenylation, and RNA editing, thereby shaping the RNA identity of nervous system cells. Recent evidence suggests that interactions between RNA-binding proteins and cis-regulatory elements such as promoters and enhancers play a role in the determination of neuron-specific expression profiles. Here, we discuss possible mechanisms through which transcription and RNA processing cross-talk to generate the uniquely complex neuronal transcriptome, with a focus on alternative 3′-end formation

Authors: Zukher, I; Dujardin, G; Sousa-Luís, R; Proudfoot, NJ

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Nature Research
• Journal: Nature Structural & Molecular Biology
• Volume: 30
• Issue: 10
• Pages: 1536-1548
• Publication date: 2023-10-02
• DOI: 10.1038/s41594-023-01090-9
• EISSN: 1545-9985
• ISSN: 1545-9993
• Language: English
• Keywords: Gene; Transcription factor II D; Polyadenylation; RNA polymerase II; Transcription (linguistics); Cell biology; Promoter; Genetics; RNA polymerase; Gene expression; Biology; RNA polymerase II holoenzyme; RNA; RNA splicing